Coding
Part:BBa_K197005:Design
Designed by: Patrick Harrigan Group: iGEM09_Berkeley_Wetlab (2009-10-21)
{Hag AtD}
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]
Source
Pool O09ig121 through O09ig128, assemble by PCA PCR O09ig121/O09ig128 on PCA reaction (269, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-B09ig121 {?Hag AtD?} ---- O09ig121 PCA assembly of {?Hag AtD?} (B09ig121) CCATAGAATTCATGAGATCTAACGAAAACAAAGCTAACGCTGGTATCT O09ig122 PCA assembly of {?Hag AtD?} (B09ig121) CCTGCGGCATAGAAGCCATAGCCATAGCAGAAGAGATACCAGCGTTAGCTTTG O09ig123 PCA assembly of {?Hag AtD?} (B09ig121) TATGGCTTCTATGCCGCAGGCTTATATTCCGGGTCGTTCTATGGTTACTGGCG O09ig124 PCA assembly of {?Hag AtD?} (B09ig121) CAACAGCACCCTGACCGTTGTGGGTAGCGATACCGCCAGTAACCATAGAACGA O09ig125 PCA assembly of {?Hag AtD?} (B09ig121) CAACGGTCAGGGTGCTGTTGCTGTTGGTCTGTCTAAACTGTCTGACAACGGTC O09ig126 PCA assembly of {?Hag AtD?} (B09ig121) TGTCAGCAGAACCGTTGATTTTGAAAACCCACTGACCGTTGTCAGACAGTTTA O09ig127 PCA assembly of {?Hag AtD?} (B09ig121) AATCAACGGTTCTGCTGACACTCAAGGCCATGTAGGTGCTGCTGTTGGTGCTG O09ig128 PCA assembly of {?Hag AtD?} (B09ig121) CGTTAGGATCCGAAGTGGAAACCAGCACCAACAGCAGCACCT